Abstract
Although it has not been explicitly recognized in the literature, biotransformations in organic media can be classified as either solvent-based or solvent-free reactions. In the former systems, one or more substrates are dissolved in an inert organic solvent that does not participate in the reaction in any respect, but to provide an environment for the enzyme to exert its action on the dissolved substrate(s). In a solvent-free system, no other compounds but substrate(s) and enzyme are present in a reactor. In principle, one substrate can be used in a large excess over another and, if so, it may also act as a solvent for other reactants. Examples of such “neat” biotransformations can be found even in the early literature (1-3).
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Yamane, T. (2001). Solvent-Free Biotransformations of Lipids. In: Vulfson, E.N., Halling, P.J., Holland, H.L. (eds) Enzymes in Nonaqueous Solvents. Methods in Biotechnology, vol 15. Humana Press. https://doi.org/10.1385/1-59259-112-4:509
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DOI: https://doi.org/10.1385/1-59259-112-4:509
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