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Enzymatic Transformations in Supercritical Fluids

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Enzymes in Nonaqueous Solvents

Part of the book series: Methods in Biotechnology ((MIBT,volume 15))

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Abstract

Water is the most common solvent for biochemical reactions both in vivo and in vitro in enzymological experiments. Unfortunately, synthetic reactions that can be carried out by reversing the hydrolytic action of certain enzymes as well as other biotransformations (e.g., oxidation) are difficult or even impossible to operate in water. Oxidation reactions are limited by the poor solubility of oxygen in water, and syntheses are impeded by high water activity. In order to shift the thermodynamic equilibrium in favor of the synthesis, it is necessary to use nonaqueous solvents. However, the use of solvents can be problematic because of toxicity, flammability, and increasing environmental concerns. As a result, supercritical fluids (SCFs) have attracted much attention in recent years as an alternative to organic solvents for carrying out enzymatic reactions. Up to now, SCFs have only been used in large-scale industrial processing to extract plant materials (e.g., coffee, hops). Nevertheless, interest in the use of SCF as a solvent for biocatalysis is growing rapidly (see reviews in refs. 1-9).

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Author information

Authors and Affiliations

  1. Centre de Bioingenierie Gilbert Durand, Department de Genie Biochimique et Alimentaire, Complexe Scientifique de Rangueil, Toulouse Cedex, France

    Alain Marty

  2. Departement de Genie Biochimique et Alimentaire, Institut National des Sciences Appliquees, Toulouse Cedex, France

    Jean-Stéphane Condoret

Authors
  1. Alain Marty
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  2. Jean-Stéphane Condoret
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Editor information

Editors and Affiliations

  1. Institute of Food Research, Norwich, UK

    Evgeny N. Vulfson

  2. University of Strathclyde, Glasgow, UK

    Peter J. Halling

  3. Brock University, St. Catharines, Ontario, Canada

    Herbert L. Holland

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© 2001 Humana Press Inc.

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Marty, A., Condoret, JS. (2001). Enzymatic Transformations in Supercritical Fluids. In: Vulfson, E.N., Halling, P.J., Holland, H.L. (eds) Enzymes in Nonaqueous Solvents. Methods in Biotechnology, vol 15. Humana Press. https://doi.org/10.1385/1-59259-112-4:587

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  • DOI: https://doi.org/10.1385/1-59259-112-4:587

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-929-2

  • Online ISBN: 978-1-59259-112-1

  • eBook Packages: Springer Protocols

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