Abstract
Alkaline phosphatase (EC 3.1.3.1) from bovine intestinal mucosa is a valuable label. It is stable, has a moderate size (140 kDa), a high turnover number, and can be assayed using a variety of different substrates which are measured by changes in absorbance, fluorescence, or luminescence. The most common method of labeling immunoglobulin G (IgG) antibody with this enzyme uses the homobifunctional reagent glutaraldehyde.
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References
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© 2002 Humana Press Inc., Totowa, NJ
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Wisdom, G.B. (2002). Alkaline Phosphatase Labeling of IgG Antibody. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-169-8:343
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DOI: https://doi.org/10.1385/1-59259-169-8:343
Publisher Name: Humana Press
Print ISBN: 978-0-89603-940-7
Online ISBN: 978-1-59259-169-5
eBook Packages: Springer Book Archive