Abstract
The isolation and purification of proteoglycans derived from mineralized tissues requires sequential dissociative extraction and fractionation techniques that were first employed on developing dental enamel (1) and then on bone (2). The basic method involves preliminary extraction in nondemineralizing denaturing buffers, followed by extraction with demineralizing and denaturing solutions combined. The denaturing buffer extract is considered to be the osteoid-associated proteoglycan pool, while the demineralizing buffer extract is taken as the mineral-associated pool of proteoglycans. Following extraction procedures, the samples are subjected to anionexchange chromatography and size-exclusion chromatography for purification.
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© 2001 Humana Press Inc., Totowa, NJ
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Fedarko, N.S. (2001). Purification of Proteoglycans from Mineralized Tissues. In: Iozzo, R.V. (eds) Proteoglycan Protocols. Methods in Molecular Biology™, vol 171. Humana Press. https://doi.org/10.1385/1-59259-209-0:019
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DOI: https://doi.org/10.1385/1-59259-209-0:019
Publisher Name: Humana Press
Print ISBN: 978-0-89603-759-5
Online ISBN: 978-1-59259-209-8
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