Abstract
As the Human Genome Project continues toward its goal of characterizing the genome of human and selected model organisms through complete mapping and sequencing of their DNA, unique opportunities are becoming available for studying genetic variation in humans and its relationship with disease risk and aging. Consequently, new techniques have been invented to rapidly screen genes for biological information.
Certain commercial equipment or materials are identified in this chapter to adequately specify experimental procedures. Such identification does not imply endorsement by the National Institute of Standards and Technology, nor does it imply that the materials or equipment identified are necessarily the best available for the purpose.
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References
Gillespie, D. and Spiegelman, S. A. (1965) A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J. Mol. Biol. 12, 829–842.
Ritossa, F., Malva, C., Boncinelli, E., Graziani, F., and Polito, L. (1971) The first steps of magnification of DNA complementary to ribosomal RNA in Drosophila melanogaster. Proc. Nat. Acad. Sci. USA 68, 1580–1584.
Sinibaldi, R. M. and Cummings, M. R. (1981) Localization and characterization of rDNA in Drosophila tumiditarsus. Chromosoma 81, 655–671.
Kafatos, F. C., Jones, C. W., and Estradiatis, A. (1979) Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure. Nucleic Acids Res. 24, 1541–1552.
Lockhart, D. J., Dong, H., Byrne, M. C., Follettie, M. T., Gallo, M. V., Chee, M. S., Mittmann, M., Wang, C., Kobayashi, M., Horton, H., and Brown, E. L. (1996) Expression monitoring by hybridization to high-density oligonucleotide arrays. Nat. Biotechnol. 14, 1675–1680.
Schena, M, Shalon, D., Davis, R. W., and Brown, P. O. (1995) Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science 270, 467–470.
Guo, Z., Guilfoyle, R. A., Thiel, A. J., Wang, R., and Smith, L. M. (1994) Direct fluorescence analysis of genetic polymorphisms by hybridization with oligonucleotide arrays on glass supports. Nucleic Acids Res. 22, 5456–5465.
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© 2001 Humana Press Inc.
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Sinibaldi, R., O’Connell, C., Seidel, C., Rodriguez, H. (2001). Gene Expression Analysis on Medium-Density Oligonucleotide Arrays. In: Rampal, J.B. (eds) DNA Arrays. Methods in Molecular Biology™, vol 170. Humana Press. https://doi.org/10.1385/1-59259-234-1:211
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DOI: https://doi.org/10.1385/1-59259-234-1:211
Publisher Name: Humana Press
Print ISBN: 978-0-89603-822-6
Online ISBN: 978-1-59259-234-0
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