Abstract
Evaluation of antiviral agents against Epstein-Barr virus (EBV) has been hampered by the lack of a permissive cell system for the replication of this virus. The extent of EBV replication detected in producer cell lines (P3HR-1 and B95-8) is limited: only a small fraction (<10%) of the cell population spontaneously produces virus at any given time. Attempts to increase the virus yield by manipulation of the culture conditions, such as temperature shifts, exposure to halogenated pyrimidines, such as BUdR and IUdR, and X-ray, have not been very successful. Superinfection of Raji (a nonvirus-producer line) cells with P3HR-1 virus enhances EBV replication and provides a more efficient system for evaluation of antiviral drugs. However, this method requires infection with virus isolated from a large quantity of culture fluids of P3HR-1 cells The virus yield varies from preparation to preparation. In addition, the system is not practical for large-scale drug screening.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
zur Hausen, H., O’Neill, F. J., and Freeze, J. K. (1978) Persisting oncogenic herpesvirus induced by the tumor promoter TPA. Nature (London) 272, 373–375.
Lin, J.C., Shaw, J. E., Smith, M. C., and Pagano, J. S. (1979) Effect of 12-O-tetradecanoyl-phorbol-13-acetate on the replication of Epstein-Barr virus. I. Characterization of viral DNA. Virology 99, 183–187.
Lin, J. C., Smith, M. C., and Pagano, J. S. (1982) Effects of 12-O-tetra-decanoyl-phorbol-13-acetate on cell proliferation and Epstein-Barr virus DNA replication. Virology 117, 186–194.
Lin, J. C., Smith, M. C., and Pagano, J. S. (1983) Activation of latent Epstein-Barr virus genomes: selective stimulation of synthesis of chromosomal proteins by a tumor promoter. J. Virol. 45, 985–991.
Mar, E. C. and Lin, J. C. (1996) Effects of temporal treatments of nucleoside analogs on replication of Epstein-Barr virus. Ninth International Conference on Antiviral Research, Fukushima, Japan, May 19–24.
Lin, J. C., Smith, M. C., and Pagano, J. S. (1984) Prolonged inhibitory effect of 9-(1,3)-dihydroxy-2-propoxymethyl)guanine against replication of Epstein-Barr virus. J. Virol. 50, 50–55.
Lin, J. C., Zhang, Z. X., Chao, T. C., Sim, I., and Pagano, J. S. (1989) Synergistic inhibition of Epstein-Barr virus transformation of B lymphocytes by a and r interferon and by 3′-azido-3′-deoxythymidine. J. Infect. Dis. 159, 248–254.
Reed, L. J. and Muench, H. (1938) A simple method of estimating fifty percent endpoints. Am. J. Hygiene 27, 493–497.
Nonoyama, M. and Pagano, J. S. (1971) Detection of Epstein-Barr viral genome in nonproductive cells. Nature (London) New Biol. 233, 103–106.
Burton, K. (1956) A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of DNA. Biochem. J. 62, 315–319.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Humana Press Inc.
About this protocol
Cite this protocol
Lin, JC. (2000). Strategies for Evaluation of Antiviral Agents Against Epstein-Barr Virus in Culture. In: Kinchington, D., Schinazi, R.F. (eds) Antiviral Methods and Protocols. Methods in Molecular Medicine™, vol 24. Humana Press. https://doi.org/10.1385/1-59259-245-7:139
Download citation
DOI: https://doi.org/10.1385/1-59259-245-7:139
Publisher Name: Humana Press
Print ISBN: 978-0-89603-561-4
Online ISBN: 978-1-59259-245-6
eBook Packages: Springer Protocols