Abstract
It is well known that the superoxide anion radical (O-2) is produced in the body as a result of biochemical activities of many cells, such as neutrophils, macrophages, and Kupffer cells (1). Production of O-2 is detectable by various methods, including cytochrome-c oxidation (1), nitroblue tetrazolium (NBT) staining, and chemiluminescence (CL) measurement (2). The site and amount of O-2 production is variable with time and readily determined in vivo by applying the CL method. In earlier studies, the CL method was successfully applied for detection of O-2 generation at a tissue level in the lung (3), liver (4), pancreas (5), heart (6), and brain (7). Combined with video imaging techniques, the CL method makes it possible to analyze O-2 generation at a high spatial resolution, sometimes even at a single cell level. In this chapter, we describe the CL method applied to O-2 detection from the islets of Langerhans in the perfused pancreas.
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© 2002 Humana Press Inc.
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Sakurai, T., Terakawa, S. (2002). Superoxide Production in the Islet of Langerhans Detected by the MCLA Chemiluminescence Method. In: Armstrong, D. (eds) Oxidants and Antioxidants. Methods in Molecular Biology™, vol 196. Humana Press. https://doi.org/10.1385/1-59259-274-0:203
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DOI: https://doi.org/10.1385/1-59259-274-0:203
Publisher Name: Humana Press
Print ISBN: 978-0-89603-851-6
Online ISBN: 978-1-59259-274-6
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