Abstract
In this chapter, methods will be reviewed for the identification of the type of modified amino acid residue (serine, threonine, or tyrosine) and position in the sequence of a phosphopeptide.
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References
Walsh, K. A. and Sasagawa, T. (1986) High performance liquid chromatography probes for post-translationally modified amino acids. Methods Enzymol. 106, 22–29.
Carlomagno, L., Huebner, V. D., and Matthews, H. R. (1985) Rapid separation of phosphoamino acids including the phosphohistidines by isocratic high-performance liquid chromatography of the orthophthalaldehyde derivatives. Anal. Biochem. 149, 344–348.
Coull, J. M., Dixon, J. D., Laursen, R. A., Koster, H., and Pappin, D. (1989) Development of membrane supports for the solid phase sequence analysis of proteins and peptides, in Methods in Protein Sequence Analysis (Wittmann-Liebold, B., ed.), Springer-Verlag, Berlin, pp. 69–78.
Meyer, H. E., Hoffmann-Posorske, E., Korte, H., and Heilmeyer, L. M. G., Jr.(1986) Sequence analysis of phosphoserine-containing peptides. Modification for picomolar sensitivity. FEBS Lett. 204, 61–66.
Kemp, B. E. (1980) Relative alkali stability of some peptide o-phosphoserine and o-phosphothreonine esters. FEBS Lett. 110, 308–312.
Martensen, T. M. (1982) Phosphotyrosine in proteins. Stability and quantification. J. Biol. Chem. 257, 9848–9852.
Holmes, C. F. B. (1987) A new method for the selective isolation of phosphoserine-containing peptides. FEBS Lett. 215, 21–24.
Bean, M. F., Annan, R. S., Hemling, M. E., Mentzer, M. Huddleston, M. J., and Carr, S. A. (1995) LC-MS methods for selective detection of posttranslational modifications in proteins: glycosylation, phosphorylation, sulfation and acylation, in Techniques in Protein Chemistry VI (Crabb, J. W., ed.), Academic Press, San Diego, pp. 107–116.
Aitken, A., Patel, Y., Martin, H., Jones, D., Robinson, K., Madrazo, J., and Howell, S. (1994) Electrospray mass spectrometric analysis with on-line trapping of posttranslationally modified mammalian and avian brain 14-3-3 isoforms. J. Prot. Chem. 13, 463–65.
Bartlet-Jones, M., Jeffery, W. A. Hansen, H. F., and Pappin, D. J. C. (1994) Peptide ladder sequencing by mass spectrometry using a novel, volatile degradation reagent. Rapid Comm. Mass Spec. 8, 737–742.
Hunter, T. and Sefton, B. M. (1980) Transforming gene product of Rous sarcoma virus phosphorylates tyrosine. Proc. Nat. Acad. Sci. USA 77, 1311–1315.
Yang, J. C., Fujitaki, J. M., and Smith, R. A. (1982) Separation of phospho-hydroxyamino acids by high performance liquid chromatography. Anal. Biochem. 122, 360–363.
Capony, J-P. and Demaille, J. G. (1983) A rapid microdetermination of phosphoserine, phosphothreonine and phosphotyrosine in proteins by automatic cation exchange on a conventional amino acid analyser. Anal. Biochem. 128, 206–212.
Morrice, N. and Aitken, A. (1985) A simple and rapid method of quantitative analysis of phosphoamino acids by high performance liquid chromatography. Anal. Biochem. 148, 207–212.
Amess,B., Manjarrez-Hernandez, H. A., Howell, S. A., Learmonth, M. P., and Aitken, A. (1992) Multisite phosphorylation of the 80kDa (MARCKS) protein kinase C substrate in C3H/10T1/2 fibroblasts. Quantitative analysis of individual sites by solid-phase microsequencing. FEBS Lett. 297, 285–291.
Pisano, A., Packer, N. H., Redmond J. W., Williams, K. L., and Gooley, A. A. (1995) In Methods in Protein Structure Analysis (Atassi, M. Z. and Appella, E., eds.), Plenum Press, New York, p. 69.
Wang, Y. H., Fiol, C. J., DePaoli-Roach, A. A., Bell, A. W., Hermodson, M. A., and Roach, P. J. (1988) Identification of phosphorylation sites in peptides using a gas-phase sequencer. Anal. Biochem. 174, 537–547.
Annan, W. D., Manson, W., and Nimmo, J. A.(1982) The identification of phosphoseryl residues during the determination of amino acid sequence in phos-phoproteins. Anal. Biochem. 121, 62–68.
Richardson, W. S., Munksgaard, E. C., and Butler, W. T. (1978) Rat incisor phos-phoprotein. The nature of the phosphate and quantitation of the phosphoserine. J. Biol. Chem. 253, 8042–8046.
Downs, F., Peterson, C., Murty, V. L. N., and Pigman, W. (1977) Quantitation of the beta-elimination reaction as used on glycoproteins. Int. J. Peptide Protein Res. 10, 315–322.
Hunt, D. F., Yates, J. R., Shabanowitz, J., Winston, S., and Hauer, C. R. (1986) Protein sequencing by tandem mass spectrometry. Proc. Natl. Acad. Sci. USA 83, 6233–6237.
Hendrickson, C. L. and Emmett, M. R. (1999) Electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry. Ann. Rev. Phys. Chem. 50, 517–536.
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Aitken, A., Learmonth, M. (2003). Analysis of Sites of Protein Phosphorylation. In: Smith, B.J. (eds) Protein Sequencing Protocols. Methods in Molecular Biology™, vol 211. Humana Press. https://doi.org/10.1385/1-59259-342-9:385
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DOI: https://doi.org/10.1385/1-59259-342-9:385
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Print ISBN: 978-0-89603-975-9
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