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Identification and Differentiation of Brucella abortus Field and Vaccine Strains by BaSS-PCR

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PCR Detection of Microbial Pathogens

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 216))

Abstract

Brucellosis is a bacterial disease affecting livestock worldwide. Historically, at least seven species of pathogenic Brucella have been described, based primarily on host preference. Genetically, it appears that there is only a single species with host-adapted strains (1). Brucella abortus typically infects cattle and causes abortion. It is easily spread within a herd and can cause significant economic loss. It is also zoonotic, causing a range of chronic symptoms in humans. Many countries have developed a brucellosis eradication program and have significantly reduced or eliminated the disease, thanks in part to the use of two live-vaccines administered in early calfhood, B. abortus strain 19 and strain RB51. These are the most commonly used vaccines for cattle.

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References

  1. Verger, J. M., Grimont, F., Grimont, P. A. D., and Grayon, M. (1987) Taxonomy of the genus Brucella. Ann. Inst. Pasteur/Microbiol. 138, 235–238.

    Article  CAS  Google Scholar 

  2. Bricker, B. J., and Halling S. M. (1994) Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis and Brucella suis bv. 1 by PCR. J. Clin. Microbiol. 32, 2660–2666.

    PubMed  CAS  Google Scholar 

  3. Bricker, B. J., and Halling S. M. (1995) Enhancement of the Brucella AMOS PCR assay for differentiation of Brucella abortus vaccine strains S19 and RB51. J. Clin. Microbiol. 33, 1640–1642.

    PubMed  CAS  Google Scholar 

  4. Ewalt, D. R., and Bricker, B. J. (2000) Validation of the abbreviated AMOS PCR as a rapid screening method for differentiation of Brucella abortus field strain isolates and the vaccine strains, 19 and RB51. J. Clin. Microbiol. 38, 3085–3086.

    PubMed  CAS  Google Scholar 

  5. Vemulapalli, R., McQuiston, J. R., Schurig, G. G., Sriranganathan, N., Halling, S. M., and Boyle, S. M. (1999) Identification of an IS711 element interrupting the wboA gene of Brucella abortus vaccine strain RB51 and a PCR assay to distinguish strain RB51 from other Brucella species and strains. Clin. Diagn. Lab. Immunol. 6, 760–764.

    PubMed  CAS  Google Scholar 

  6. Sangari, F. J., and Agüero, J. (1994) Identification of Brucella abortus B19 vaccine strain by the detection of DNA polymorphism at the ery locus. Vaccine 12, 435–438.

    Article  PubMed  CAS  Google Scholar 

  7. Sangari, F. J., Garcia-Lobo, J. M., and Agüero, J. (1994) The Brucella abortus vaccine strain B19 carries a deletion in the erythritol catabolic genes. FEMS Microbiol. Lett. 121, 337–342.

    Article  PubMed  CAS  Google Scholar 

  8. Prince, A. M., and Andrus, L. (1992) PCR: How to kill unwanted DNA. BioTechniques 12, 358–360.

    PubMed  CAS  Google Scholar 

  9. Alton, G. G., Jones, L. M., Pietz, D. E. (1975) Laboratory Techniques in Brucellosis 2nd ed., World Health Organization Monograph Series, No. 55, World Health Organization, Geneva, pp. 11–13.

    Google Scholar 

  10. Alton, G. G., Jones, L. M., Angus, R. D. and Verger, J. M. (1988) Bacteriological methods, in Techniques for the Brucellosis Laboratory. INRA, Paris, pp. 13–62.

    Google Scholar 

  11. Mayfield, J. E., Bantle, J. A., Ewalt, D. R., Meador, V. P., and Tabatabai, L. B. ( 1990) Detection of Brucella cells and cell components, in Animal Brucellosis, (Nielsen, K. and Duncan, J. R., ), CRC Press, Boca Raton, FL, USA, pp. 97–120.

    Google Scholar 

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© 2003 Humana Press Inc., Totowa, NJ

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Ewalt, D.R., Bricker, B.J. (2003). Identification and Differentiation of Brucella abortus Field and Vaccine Strains by BaSS-PCR. In: Sachse, K., Frey, J. (eds) PCR Detection of Microbial Pathogens. Methods in Molecular Biology™, vol 216. Humana Press. https://doi.org/10.1385/1-59259-344-5:97

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  • DOI: https://doi.org/10.1385/1-59259-344-5:97

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-049-6

  • Online ISBN: 978-1-59259-344-6

  • eBook Packages: Springer Protocols

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