Abstract
Lentivirus vectors are expected to become excellent tools for gene transfer in gene therapy and functional genomics and for engineering transgenic animals. When fully developed, their use will cover a whole gamut of endeavors, from discovery to therapeutics. Because they integrate into the host cell genome and become a permanent member of the genetic makeup of the recipient cell, retroviruses are well suited for stable expression of the transgenes. This property is shared by all retroviruses, including lentiviruses (1). Apart from the poor suitability of the conventional retroviral vectors for human gene therapy, what brought the prospects of lentiviral vectors to the forefront was their ability to cause gene transfer into nondividing, quiescent cells as well as dividing cells (2). This attribute will become increasingly important as more knowledge is gained about the biology of stem cells and a more optimized technology is developed for transplantation of genetically modified cells.
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Notes
- 1.
*
The HIV-2 vectors used in our laboratory are derived from nonpathogenic strain ST
References
Miller, A. D. (1997) Development and application of retroviral vectors, in Retroviruses (Coffin, J. M., Huges, S. H., and Varmus, H. E., eds.), Cold Spring Harbor Laboratory, Cold Spring Harbor, N Y, pp. 437–473.
Naldini, L., Blomer, U., Gallay, P., et al. (1996) In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science 272, 263–267.
Fletcher, T.M., Brichacek, B., Sharova, N., et al. (1996) Nuclear import and cell cycle arrest functions of the HIV-1 Vpr protein are encoded by two separate genes in HIV-2/SIVsm. EMBO J. 15, 6155–6165.
Lois, C., Hong, E. J., Pease, S., Brown, E. J., and Baltimore, D. (2002) Germ line transmission and tissue-specific expression of transgenes delivered by lentiviral vectors. Science 295, 868–872.
Pfeifer, A., Ikawa, M., Dayn, Y., and Verma, I. M. (2002) Transgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos. Proc. Natl. Acad. Sci. USA 99, 2140–2145.
Arya, S. K., Zamani, M., and Gallo, R. C. (1994) HIV-2 inhibits HIV-1. AIDS Res. Hum. Retroviruses 10, 515S.
Arya, S. K. and Gallo, R. C. (1996) Human immunodeficiency virus (HIV) type 2 mediated inhibition of HIV type 1: a new approach to gene therapy of HIV infection. Proc. Natl. Acad. Sci. USA 93, 4486–4491.
Sadaie, M. R., Zamani, M., Whang, S., Sistron, N., and Arya, S. K. (1998) Towards developing HIV-2 lentivirus-based retroviral vectors for gene therapy: dual gene expression in the context of HIV-2 LTR and Tat. J. Med. Virol. 54, 118–128.
Garzino-Demo, A., Gallo, R. C., and Arya, S. K. (1995) Human immunodeficiency virus type 2 (HIV-2): packaging signal and associated negative regulatory element. Hum. Gene Ther. 6, 177–184.
McBride, M. S., Schwarts, M. D., and Panganiban, A. T. (1997) Efficient encapsidation of human immunodeficiency virus type 1 vectors and further characterization of cis elements required for encapsidation. J. Virol. 71, 4544–4554.
McCann, E. M. and Lever, A. M. (1997) Location of cis-acting signals important for RNA encapsidation in the leader sequence of human immunodeficiency virus type 2. J. Virol. 71, 4133–4137.
Poeschla, E., Gilbert, J., Huang, S., Ho, A., and Wong-Staal, F. (1998) Identification of a human immunodeficiency virus type 2 (HIV-2) encapsidation determinant and transduction of nondividing human cells by HIV-2 based lentivirus vectors. J. Virol. 72, 6527–6536.
Arya, S. K., Zamani, M., and Kundra, P. (1998) HIV-2 lentivirus vectors for gene transfer: expression and potential for helper virus free packaging. Hum. Gene Ther. 9, 1371–1380.
Malim, M. H. and Cullen, B. R. (1993) Rev and the fate of pre-mRNA in the nucleus: implications for the regulation of RNA processing in eukaryotes. Mol. Cell. Biol. 13, 6180–6189.
Schneider, R., Campbell, M., Nasioulas, G., Felber, B. K., and Pavlakis, G. N. (1997) Inactivation of the human immunodeficiency virus type 1 inhibitory elements allow Rev-independent expression of Gag and Gag/Protease and particle formation. J. Virol. 7, 4892–4903.
Zennou, V., Petit, C., Guetard, D., Nerhbass, U., Montagnier, L, and Charnau, P. (2000) HIV-1 genome nuclear import is mediated by a central DNA flap. Cell 101, 173–185.
D’Costa, J., Brown, H. M., Kundra, P., Davis-Warren, A., and Arya, S. K. (2001) Human immunodeficiency virus type 2 lentiviral vectors: packaging signal and splice donor in expression and encapsidation. J. Gen. Virol. 82, 425–434.
Cui, Y., Iwakuyma, T., and Chang, L. (1999) Contribution of viral splice sites and cis-regulatory elements to lentivirus vector function. J. Virol. 73, 6171–6176.
Zufferey, R., Nagy, D., Mandel, R. J., Naldini, L, and Torno, D. (1997) Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo. Nat. Biotech. 15, 871–875.
Wu, X., Wakefield, J. K., Hongmei, L., Xiao, H., Kralovics, J. T., and Kapes, J. C. (2000) Development of a novel trans-lentiviral vector that affords predictable safety. Mol. Ther. 2, 47–55.
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Arya, S.K., Cho, J.E., Chang, S.H. (2003). HIV-2 Vectors. In: Federico, M. (eds) Lentivirus Gene Engineering Protocols. Methods in Molecular Biology™, vol 229. Humana Press. https://doi.org/10.1385/1-59259-393-3:219
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DOI: https://doi.org/10.1385/1-59259-393-3:219
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