Abstract
Incremental truncation is a method for creating a library of every one base truncation of dsDNA. Incremental truncation libraries can be created by time dependent Exo III digestions (1) or by the incorporation of α-phosphorothioate dNTPs (αS-dNTPs) (2). The fusion of two incremental truncation libraries is called an ITCHY library (3). An ITCHY library created from a single gene consists of genes with internal deletions and duplications. An ITCHY library created between two different genes consists of gene fusions created in a DNA-homology independent fashion. ITCHY libraries, as well as an incremental truncation-like method called SHIPREC (4), have the potential to create proteins with improved or novel properties as well as to generate artificial families for in vitro recombination in a method called SCRATCHY (5) (see Chapter 17).
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References
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© 2003 Humana Press Inc., Totowa, NJ
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Ostermeier, M., Lutz, S. (2003). The Creation of ITCHY Hybrid Protein Libraries. In: Arnold, F.H., Georgiou, G. (eds) Directed Evolution Library Creation. Methods in Molecular Biology™, vol 231. Humana Press. https://doi.org/10.1385/1-59259-395-X:129
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DOI: https://doi.org/10.1385/1-59259-395-X:129
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