Abstract
Electrophoresis is an important and fundamental tool for DNA analysis. Traditional DNA electrophoresis is labor-intensive, skill-dependent, and relatively slow. To achieve greater performance, capillary electrophoresis (CE) has been developed for DNA work (1–2). The DNA fragments can be separated by molecular sieving in CE with gel-filled capillaries, based on the same principle as that of the conventional gel electrophoresis. The whole procedure can be completed in a relatively short period with great resolution. Recently, CE equipped with laser-induced fluorescence (CE-LIF) has been used as a tool for DNA analysis (3). Using fluorescence DNA intercalators in a gel-filled capillary, the detection sensitivity was found to increase 2–3 orders of magnitude over that of UV detection, and reaches picogram levels (3).
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References
Strege, M. and Lagu, A. (1991) Separation of DNA restriction fragments by capillary electrophoresis using coated fused silica capillaries. Anal. Chem. 63, 1233–1236.
Ulfelder, K. J., Schwartz, H. E., Hall, J. M., and Sunnzeri, F. J. (1992) Restriction fragment length polymorphism analysis of ERBB2 oncogene by capillary electrophoresis. Anal. Biochem. 200, 260–267.
Schwartz, H. E. and Ulfelder, K. J. (1992) Capillary electrophoresis with laser induced fluorescence detection of PCR fragments thiazole orange. Anal. Chem. 64, 1737–1740.
Rolfs, A., Schuller, L., Finckh, U., Weber-Rolfs, I., eds. (1992) PCR: Clinical Diagnostics and Research. Springer-Verlag, Berlin.
Liu, M. S., Zang, J., Evangelista, R. A., Rampal, S., and Chen, F. T. A. (1995) Double-stranded DNA analysis by capillary electrophoresis with laser-induced fluorescence using ethidium bromide as an intercalator. BioTechniques 18, 316–323.
Pao, C. C., Kao, S. M., Hor, J. J., and Chang, S. Y. (1993) Lack of mutational alteration in the conserved regions of ZFY and SRY gene of 46, XY females with gonadal dysgenesis. Hum. Reprod. 8, 224–228.
Hixson, J. E. and Vernier, D. T. (1990) Restriction isotyping of human apolipo-protein E by gene amplification and cleavage with HhaI. J. Lipid Res. 31, 545–548.
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© 1999 Humana Press Inc.
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Liu, MS., Chen, FT.A. (1999). Rapid Analysis of Amplified Double-Stranded DNA by Capillary Electrophoresis with Laser-Induced Fluorescence Detection. In: Palfrey, S.M. (eds) Clinical Applications of Capillary Electrophoresis. Methods in Molecular Medicine™, vol 27. Humana Press. https://doi.org/10.1385/1-59259-689-4:121
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DOI: https://doi.org/10.1385/1-59259-689-4:121
Publisher Name: Humana Press
Print ISBN: 978-0-89603-639-0
Online ISBN: 978-1-59259-689-8
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