Abstract
The apolipoproteins associated with serum lipoprotein particles give structural stability as well as regulatory control in lipid metabolism. The development of atherosclerosis is linked to dysfunction in lipid metabolism, and the serum lipoproteins are directly involved, either through the action of their apolipoprotein components or in combination with the lipids sequestered in these particles. Consequently, quantitative features of the apolipoproteins (apos), serum levels, distribution within the lipoprotein population, and the appearance of isoforms, are potential markers for cardiovascular disease risk that are being added to the lipid profile for more effective cardiovascular risk screening (1). Currently, apo quantitation is being carried out using immunoassay or gel electrophoresis for separation and staining with image analysis for quantitation (2). Capillary electrophoresis has the potential for higher resolution, greater specificity, speed, and automation, coupled with on-line detection for more accurate and precise quantitation.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Mahley, R. W., Innerarity, T. L., Rail, S. C., Jr., and Weisgraber, K. H. (1984) Plasma lipoproteins: apoprotein structure and function. J. Lipid Res. 25, 1277ā1294.
Bergeron, N., Kotite, L., and Havel, R. J. (1996) Simultaneous quantification of apolipoproteins B-i 00, B-48, and E separated by SDS-PAGE. Methods Enzymol. 263, 82ā94.
Cruzado, I. D., Hu, A. Z., and Macfarlane, R. D. (1996) Influence of dodecyl sulfate ions on the electrophoretic mobilities of lipoprotein particles measured by HPCE. J. Capillary Electrophoresis 3, 25ā29.
Cruzado, I. D., Song, S., Crouse, S. F., and OāBrien, B. C. (1996) Characterization and quantitation of the apoproteins of high-density lipoprotein by capillary electrophoresis. Anal. Biochem. 243, 100ā109.
Macfarlane, R. D., Bondarenko, P. V., Cockrill, S. L., Cruzado, I. D., Koss, W., McNeal, C. J., Spiekernian, A. M., and Watkins, L. K. (1997) Development of a lipoprotein profile using capillary electrophoresis and mass spectrometry. Electrophoresise 18, 1796ā1806.
Cruzado, I. D., Cockrill, S. L., McNeal, C. J., and Macfarlane, R. D. (1998) Characterization and quantitation of apolipoprotein B-100 by capillary electrophoresis. J. Lipid Res. 39, 205ā217.
Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Garmer, F. H., Provenzano, M. D., et al. (1985) Measurement of protein using bicinchoninic acid. Anal. Biochem. 150, 76ā85.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
Ā© 1999 Humana Press Inc.
About this protocol
Cite this protocol
Watkins, L.K., Cockrill, S.L., Macfarlane, R.D. (1999). Serum Apolipoproteins. In: Palfrey, S.M. (eds) Clinical Applications of Capillary Electrophoresis. Methods in Molecular Medicineā¢, vol 27. Humana Press. https://doi.org/10.1385/1-59259-689-4:99
Download citation
DOI: https://doi.org/10.1385/1-59259-689-4:99
Publisher Name: Humana Press
Print ISBN: 978-0-89603-639-0
Online ISBN: 978-1-59259-689-8
eBook Packages: Springer Protocols