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Automated vs Manual Profiling of Peptide Libraries by Mass Spectrometry

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HPLC of Peptides and Proteins

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 251))

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Abstract

Combinatorial chemistry (17) has evolved from the synthesis of very large mixtures to the preparation of collections of isolated compounds by parallel syntheses. Peptides constitute ideal materials because their preparation is completely automated using solid-phase methodology allowing high throughput syntheses.

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References

  1. Czarnik, A. W. and Dewitt, S. H. (1997) A Practical Guide to Combinatorial Chemistry. American Chemical Society, Washington, DC.

    Google Scholar 

  2. Wilson, S. R. and Czarnik, A. W. (1997) Combinatorial Chemistry—Synthesis and Application. Wiley, New York.

    Google Scholar 

  3. Bunin, B. A. (1998) The Combinatorial Index. Academic, London.

    Google Scholar 

  4. Terrett, N. K. (1998) Combinatorial Chemistry. Oxford University Press, Oxford, UK.

    Google Scholar 

  5. Gordon, E. M. and Kervin, J. F. (1998) Combinatorial Chemistry and Molecular Diversity in Drug Discovery. Wiley, New York.

    Google Scholar 

  6. Obrecht, D. and Villalgordo, J. M. (1998) Solid-Supported Combinatorial and Parallel Synthesis of Small Molecular Weight Compound Libraries. Tetrahedron Organic Chemistry Series, Vol. 17, Pergamon, Elsevier, Oxford, UK.

    Google Scholar 

  7. Jung, G. (1999) Combinatorial Chemistry—Synthesis, Analysis, Screening. Wiley-VCH, Weiheim, RFA.

    Google Scholar 

  8. Shapiro, M. J. and Wareing, J. R. (1998) NMR methods in combinatorial chemistry. Curr. Opin. Chem. Biol. 2, 372–375.

    Article  PubMed  CAS  Google Scholar 

  9. Fitch, W. L. (1999) Analytical methods for quality control of combinatorial libraries. Mol. Diversity 4, 39–45.

    Article  CAS  Google Scholar 

  10. Enjalbal, C, Martinez, J., and Aubagnac, J-L. (2000) Mass spectrometry in combinatorial chemistry. Mass Spectrom. Rev. 19,139–161.

    Article  PubMed  CAS  Google Scholar 

  11. Bray, A. M., Jhingran, A. G., Valerio, R. M., and Maeji, N. J. (1994) Simultaneous multiple synthesis of peptide amides by the multipin method—Application of vapor-phase ammonolysis. J. Org. Chem. 59, 2197–2203.

    Article  CAS  Google Scholar 

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© 2004 Humana Press Inc.

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Aubagnac, JL., Enjalbal, C., Martinez, J., Sanchez, P., Subra, G. (2004). Automated vs Manual Profiling of Peptide Libraries by Mass Spectrometry. In: Aguilar, MI. (eds) HPLC of Peptides and Proteins. Methods in Molecular Biology™, vol 251. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-742-4:377

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  • DOI: https://doi.org/10.1385/1-59259-742-4:377

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-977-3

  • Online ISBN: 978-1-59259-742-0

  • eBook Packages: Springer Protocols

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