Abstract
Quantitative polymerase chain reaction (Q-PCR) allows for the accurate and reproducible determination of the amount of target DNA in a sample through the measurement of PCR product accumulation in “real time.” This method determines starting target DNA quantity over a large assay dynamic range and requires no post-PCR sample manipulation. When used in combination with the method of chromatin immunoprecipitation (ChIP), the amount of protein binding to a specific region of DNA can be accurately and rapidly determined. A method for quantifying the presence of acetylated histones H3 and H4 on different regions of a target locus using Q-PCR after ChIP is described.
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© 2004 Humana Press Inc., Totowa, NJ
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Irvine, R.A., Hsieh, CL. (2004). Q-PCR in Combination With ChIP Assays to Detect Changes in Chromatin Acetylation. In: Tollefsbol, T.O. (eds) Epigenetics Protocols. Methods in Molecular Biology™, vol 287. Humana Press. https://doi.org/10.1385/1-59259-828-5:045
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DOI: https://doi.org/10.1385/1-59259-828-5:045
Publisher Name: Humana Press
Print ISBN: 978-1-58829-336-7
Online ISBN: 978-1-59259-828-1
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