Summary
The molecular engineering of recombinant plasmid DNA clones containing the full-length and replication-competent feline foamy (retro)virus (FFV) proviral genome is described. The methods used to combine subgenomic FFV DNA fragments can be applied to other retrovirus genomes, resulting in full-length, bacterially cloned retroviruses. In addition, techniques used to determine the replication competence of the cloned viral genomes are described. Alternative technologies (not described here) are also discussed, together with the application of cloned genomes in modern molecular virology; for example, in functional genetic studies or as starting material for retrovirus vector development.
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Acknowledgments
Part of the presented data is reprinted from ref. 3 with permission from Elsevier. We thank Patrizia Bastone, Nadine Kirchner, and Fabian Romen for critically reading the manuscript, and Lutz Gissmann for support.
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Wirtz, R., Löchelt, M. (2005). Construction and Analysis of Genomic, Full-Length Infectious Foamy Virus DNA Clones. In: Zhu, T. (eds) Human Retrovirus Protocols. Methods in Molecular Biology™, vol 304. Humana Press. https://doi.org/10.1385/1-59259-907-9:423
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DOI: https://doi.org/10.1385/1-59259-907-9:423
Publisher Name: Humana Press
Print ISBN: 978-1-58829-495-1
Online ISBN: 978-1-59259-907-3
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