Abstract
Mast cells are highly responsive cells that are capable of secreting a variety of inflammatory mediators, including histamine, heparin, serine proteases, leukotrienes, prostaglandins, and thromboxanes. Studies from several laboratories have demonstrated that mast cells have the capacity to produce a variety of cytokines in response to various stimuli. Characterization of the cytokine profiles in mast cells has routinely been determined by the performance of individual enzyme-linked immunosorbent assays. This process is expensive, time-consuming, and requires a great deal of material to characterize multiple cytokines. In this chapter, we describe a multiplex cytokine assay to detect 17 cytokines simultaneously in 50 µL of culture supernatant derived from stimulated human cord blood-derived mast cells.
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Breuel, K.F., Keith De Ponti, W. (2006). Measurement of Mast Cell Cytokine Release by Multiplex Assay. In: Krishnaswamy, G., Chi, D.S. (eds) Mast Cells. Methods in Molecular Biology, vol 315. Humana Press. https://doi.org/10.1385/1-59259-967-2:217
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DOI: https://doi.org/10.1385/1-59259-967-2:217
Publisher Name: Humana Press
Print ISBN: 978-1-58829-374-9
Online ISBN: 978-1-59259-967-7
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