Abstract
Nuclear transplantation in amphibia started in 1952. By this is meant, sexually mature cloned frogs can be obtained from the nuclei of embryo cells, differentiating cells, and larval-differentiated cells. Transplanted nuclei are reprogrammed to entirely new patterns of gene expression. In this chapter, the methods used to transplant living nuclei into enucleated eggs of Xenopus are described. A method also is described for transplanting multiple somatic cell nuclei into nonenucleated oocytes, a procedure that achieves reprogramming of gene expression in the absence of cell division.
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© 2006 Humana Press Inc.
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Gurdon, J.B. (2006). Nuclear Transplantation in Xenopus . In: Pells, S. (eds) Nuclear Reprogramming. Methods in Molecular Biology™, vol 325. Humana Press. https://doi.org/10.1385/1-59745-005-7:1
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DOI: https://doi.org/10.1385/1-59745-005-7:1
Publisher Name: Humana Press
Print ISBN: 978-1-58829-379-4
Online ISBN: 978-1-59745-005-8
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