Abstract
Embryonic stem (ES) cells represent an attractive tool not only for the study of the development of various cell types but also as a potential source of cells for transplantation. Previous studies suggested a role of the signal transduction protein SRC homology 2(SH2) protein of Beta-cells (SHB) for the development of both pancreatic β-cells and blood vessels. SHB is an SH2 domain-containing adapter protein involved in the generation of signaling complexes in response to activation of a variety of receptors, several of which have been implicated in developmental processes. Moreover, microarray analysis of ES cells expressing mutant SHB has revealed decreased expression of several genes of developmental importance. Here, we present protocols that may be used for transfection of mouse ES cells and to study the differentiation of ES cell-derived embryoid bodies (EBs) into the pancreatic β-cell lineage as well as into vascular structures with special reference to the effect of SHB. Moreover, we also provide a protocol that may be used for enrichment by fluorescenceactivated cell sorting of specific cell lineages in EBs.
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Saldeen, J. et al. (2006). The Role of the Adapter Protein SHB in Embryonic Stem Cell Differentiation Into the Pancreatic β-Cell and Endothelial Lineages. In: Turksen, K. (eds) Embryonic Stem Cell Protocols. Methods in Molecular Biology™, vol 330. Humana Press. https://doi.org/10.1385/1-59745-036-7:353
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DOI: https://doi.org/10.1385/1-59745-036-7:353
Publisher Name: Humana Press
Print ISBN: 978-1-58829-784-6
Online ISBN: 978-1-59745-036-2
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