Abstract
Agrobacterium tumefaciens is best known for its ability to transform plants by delivering the T-DNA that is processed and transferred from the resident Ti plasmid to the recipient plant cells. Less well known is the capacity of this Gram-negative bacterium to transfer its T-DNA into fungi and actinomycetes. Procedures are described on the use of the promiscuous T-DNA transfer system of A. tumefaciens to transform members of the actinomycetes. Integration of the T-DNA derivatives into the Streptomyces chromosome provides opportunities of obtaining valuable mutants of this antibiotic producing soil organism.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Bundock, P., Den Dulk-Ras, A., Beijersbergen, A., and Hooykaas, P.J.J. (1995) Trans-kingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae. EMBO J. 14, 3206–3214.
Piers, K.L., Heath, J.D., Liang, X., Stephens, K.M., and Nester, E.W. (1996) Agrobacterium tumefaciens-mediated transformation of yeast. Proc. Natl. Acad. Sci. USA 93, 1613–1618.
Shikorski, R.S., Michaud, W., Levin, H.L., Boeke, J.D., and Heiter, P. (1990) Trans-kingdom promiscuity. Nature 345, 581–582.
Bundock, P., Mróczek, K., Winkler, A.A., Steensma, H.Y., and Hooykaas, P.J.J. (1999) T-DNA from Agrobacterium tumefaciens as an efficient tool for gene targeting in Kluyveromyces lactis. Mol. Gen. Genet. 261, 115–121.
DeGroot, M.J.A., Bundock, P., Hooykaas, P.J.J., and Beijersbergen, A.G.M. (1998) Agrobacterium tumefaciens-mediated transformation of filamentous fungi. Nat. Biotechnol. 16, 839–842.
Mullins, E.D., Chen, X., Romaine, P., Gaina, R., Geiser, D.M., and Kang, S. (2001) Agrobacterium-mediated transformation of Fusarium oxysporum: an efficient tool for insertional mutagenesis and gene transfer. Phytopathology 91, 173–180.
Kelly, B. and Kado, C.I. (1997) Promiscuous gene transfer of Agrobacterium tumefaciens extends to the actinomycete Streptomyces lividans. Amer. Soc. Microbiol. 97th Annu. Mtg. Abstr. p. 417.
Kieser, T. and Hopwood, D.A. (1991) Genetic manipulation of Streptomyces: new integrating vectors and methods for gene replacement. Methods Enzymol. 204, 430–458.
Oh, S.-H. and Chater, K.F. (1997) Denaturation of circular or linear DNA facilitates targeted integrative transformation of Streptomyces coelicolor A3(2): possible relevance to other organisms. J. Bacteriol. 179, 122–127.
Mazodier, P., Petter, R., and Thompson, C. (1989) Intergeneric conjugation between Escherichia coli and Streptomyces species. J. Bacteriol. 171, 3583–3585.
Giebelhaus, L.A., Frost, L., Lanka, E., Gormley, E.P., Davies, J.E., and Leskiw, B. (1996) The Tra2 core of the IncPα plasmid RP4 is required for intergeneric mating between Escherichia coli and Streptomyces lividans. J. Bacteriol. 178, 6378–6381.
Flett, F., Mersinias, V., and Smith, C.P. (1997) High efficiency intergeneric conjugal transfer of plasmid DNA from Escherichia coli to methyl DNA restricting Streptomycetes. FEMS Microbiol. Lett. 155, 223–229.
Kelly, B.A. and Kado, C.I. (2002) Agrobacterium-mediated T-DNA transfer and integration into the chromosome of Streptomyces lividans. Mol. Plant Pathol. 3, 125–134.
Dworkin, M.M., Falkow, S., Rosenberg, E., Schleifer K.-H., and Stacebrandt, E. (eds.). (2005) The Prokaryotes, Third Edition, vol. 3, Archaea and Bacteria: Firmicutes, Actinomycetes. Springer, Heidelberg, Germany, p. 998.
Klapwijk, P.M., van Beelen, P., and Schilperoort, R.A. (1979) Isolation of a recombination deficient Agrobacterium tumefaciens mutant. Mol. Gen. Genet. 173, 171–175.
Rogowsky, P.M., Powell, B.S., Shirasu, K., et al. (1990) Molecular characterization of the vir regulon of Agrobacterium tumefaciens: complete nucleotide sequence and gene organization of the 28.63-kbp regulon cloned as a single unit. Plasmid 23, 85–106.
Zyprian, E. and Kado, C.I. (1990) Agrobacterium-mediated plant transformation by novel mini-T vectors in conjunction with a high-copy vir region helper plasmid. Plant Mol. Biol. 15, 245–256.
Kado, C.I., Heskett, M.G., and Langley, R.A. (1972) Studies on Agrobacterium tumefaciens: characterization of strains 1D135 and B6, and analysis of the bacterial chromosome, transfer RNA and ribosomes for tumor-inducing activity. Physiol. Plant Pathol. 2, 47–57.
Rogowsky, P.M., Close, T.J., Chimera, J.A., Shaw, J.J., and Kado, C.I. (1987) Regulation of the vir genes of Agrobacterium tumefaciens plasmid pTiC58. J. Bacteriol. 169, 5101–5112.
Kieser, T., Bibb, M.J., Buttner, M.J., Chater, K.F., and Hopwood, D.A. (eds.) (2000) Practical Streptomyces Genetics. The John Innes Foundation, Norwich, UK.
Hopwood, D.A., Bibb, M.J., Chater, K.F., et al. (eds.) (1985) Genetic Manipulation of Streptomyces: A Laboratory Manual, John Innes Foundation, Norwich, UK.
Gallie, D.R., Hagiya, M., and Kado, C.I. (1985) Analysis of Agrobacterium tumefaciens plasmid pTiC58 replication region with a novel high-copy-number derivative. J. Bacteriol. 161, 1034–1041.
Gallie, D.R., Zaitlin, D., Perry, K.L., and Kado, C.I. (1984) Characterization of the replication and stability regions of Agrobacterium tumefaciens plasmid pTAR. J. Bacteriol. 157, 739–745.
Southern, E.M. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503–517.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2006 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Kado, C.I., Kelly, B. (2006). Actinomycetes (Streptomyces lividans). In: Wang, K. (eds) Agrobacterium Protocols Volume 2. Methods in Molecular Biology, vol 344. Humana Press. https://doi.org/10.1385/1-59745-131-2:395
Download citation
DOI: https://doi.org/10.1385/1-59745-131-2:395
Publisher Name: Humana Press
Print ISBN: 978-1-58829-843-0
Online ISBN: 978-1-59745-131-4
eBook Packages: Springer Protocols