Skip to main content

Host-Virus Interaction: From Metagenomics to Single-Cell Genomics

  • Living reference work entry
  • First Online:
Encyclopedia of Metagenomics
  • 163 Accesses

Synonyms

DNA packaging gene; Large terminase subunit gene; TerL

Definition

dPCR (digital PCR) is a PCR reaction performed in a nanoliter or subnanoliter volume making it possible to detect single molecules.

MetaCAT (metagenome cluster analysis tool) is a metagenome data mining tool that uses an iterative dynamic clustering approach to identify the most abundant genes in a given metagenome with respect to a reference dataset containing potentially homologous genes.

Bacteriophage is a virus that infects and replicates within bacteria (phage for short).

Prophage is a phage genome that is integrated into the bacterial genome or exists in the form of a plasmid within the cell.

Introduction

It is widely appreciated today that viruses are a dominant and critical part of Earth’s biosphere. Yet despite the major advances in the study of environmental viruses in most cases, our knowledge of which viruses go with which hosts is meager. In the classic phage isolation technique, known as the plaque...

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Institutional subscriptions

References

  • Andersson AF, Banfield JF. Virus population dynamics and acquired virus resistance in natural microbial communities. Science. 2008;320(5879):1047–50.

    Article  CAS  PubMed  Google Scholar 

  • Boyd EF, Brüssow H. Common themes among bacteriophage-encoded virulence factors and diversity among the bacteriophages involved. Trends Microbiol. 2002;10(11):521–9.

    Article  CAS  PubMed  Google Scholar 

  • Casjens S. Prophages and bacterial genomics: what have we learned so far? Mol Microbiol. 2003;49(2):277–300.

    Article  CAS  PubMed  Google Scholar 

  • Daw MA, Falkiner FR. Bacteriocins: nature, function and structure. Micron. 1996;27(6):467–79.

    Article  CAS  PubMed  Google Scholar 

  • Edwards R, Rohwer F. Viral metagenomics. Nat Rev Microbiol. 2005;3(6):504–10.

    Article  CAS  PubMed  Google Scholar 

  • Kalisky T, Quake SR. Single-cell genomics. Nat Methods. 2011;8(4):311–4.

    Article  CAS  PubMed  Google Scholar 

  • Kalisky T, Blainey P, Quake SR. Genomic analysis at the single-cell level. Annu Rev Genet. 2011;45:431–45.

    Article  CAS  PubMed  Google Scholar 

  • Koonin E, Senkevich T, Dolja V. The ancient virus world and evolution of cells. Biol Direct. 2006;1(1):29.

    Article  PubMed Central  PubMed  Google Scholar 

  • Kristensen DM, Mushegian AR, Dolja VV, Koonin EV. New dimensions of the virus world discovered through metagenomics. Trends Microbiol. 2010;18(1):11–9.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Mokili JL, Rohwer F, Dutilh BE. Metagenomics and future perspectives in virus discovery. Curr Opin Virol. 2012;2(1):63–77.

    Article  CAS  PubMed  Google Scholar 

  • Nei M, Kumar S. Molecular evolution and phylogenetics. USA: Oxford University Press; 2000.

    Google Scholar 

  • Ottesen E, Hong J, Quake S, Leadbetter J. Microfluidic digital PCR enables multigene analysis of individual environmental bacteria. Science. 2006;314(5804):1464–7.

    Article  CAS  PubMed  Google Scholar 

  • Przech AJ, Yu D, Weller SK. Point mutations in exon I of the herpes simplex virus putative terminase subunit, UL15, indicate that the most conserved residues are essential for cleavage and packaging. J Virol. 2003;77(17):9613–21.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Rao VB, Feiss M. The bacteriophage DNA packaging motor. Annu Rev Genet. 2008;42:647–81.

    Article  CAS  PubMed  Google Scholar 

  • Rohwer F, Edwards R. The phage proteomic tree: a genome-based taxonomy for phage. J Bacteriol. 2002;184(16):4529–35.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Tadmor AD, Ottesen EA, Leadbetter JR, Phillips R. Probing individual environmental bacteria for viruses by using microfluidic digital PCR. Science. 2011;333(6038):58–62.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Arbel D. Tadmor .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2013 Springer Science+Business Media New York

About this entry

Cite this entry

Tadmor, A.D., Phillips, R. (2013). Host-Virus Interaction: From Metagenomics to Single-Cell Genomics. In: Nelson, K. (eds) Encyclopedia of Metagenomics. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-6418-1_792-2

Download citation

  • DOI: https://doi.org/10.1007/978-1-4614-6418-1_792-2

  • Received:

  • Accepted:

  • Published:

  • Publisher Name: Springer, New York, NY

  • Online ISBN: 978-1-4614-6418-1

  • eBook Packages: Springer Reference Biomedicine and Life SciencesReference Module Biomedical and Life Sciences

Publish with us

Policies and ethics