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Bonediol Production in Bonellia macrocarpa Hairy Root Culture

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Plant Cell and Tissue Differentiation and Secondary Metabolites

Part of the book series: Reference Series in Phytochemistry ((RSP))

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Abstract

The genus Bonellia belongs to the family Theophrastaceae, which consists of 22 species distributed in Mesoamerica, in the north and in the west of South America and the Greater Antilles. Bonellia macrocarpa (Cavanilles) Ståhl and Källersjö has generated a great interest derived from the biological activities that present the secondary metabolites of this species. Bonediol is an alkyl catechol produced by Bonellia macrocarpa with antiproliferative activity against cell lines of nasopharynx carcinoma. Hairy root culture is an alternative for study and production of bonediol. The aim of this work is to present a revision on the induction of Bonellia macrocarpa hairy root culture as an alternative strategy for bonediol production. For the production of hairy roots of B. macrocarpa, some factors have an effect on the production, such as the explant type and chemical additives on the percent of explants forming roots. The rhizogenic response of cotyledon, hypocotyl, and roots was different. For cotyledon explants, the rhizogenic response was observed from the 4th to the 6th week; however, for hypocotyl explants, the rhizogenic response was observed from the 5th to the 6th week, and finally for the root explants, the rhizogenic response was observed from the 3rd to the 4th week. Two chemical auxiliaries, glutamine and acetosyringone, had a statistically significant effect on percent of explants forming roots. The addition of 100 mg/L glutamine or 100 μM acetosyringone to the culture media generated the highest percent of explants forming roots in cotyledon; in contrast, other factors such as cysteine, dithiothreitol, and A. rhizogenes density did not have a statistically significant effect on the response. Bonediol can be produced via hairy root culture. Methanol extracts of hairy roots can produce 2.78 mg bonediol/g dried weight of transformed root, and therefore Bonellia macrocarpa hairy root culture is an alternative for bonediol production; however, it is necessary to carry out more studies that allow the scaling of the process in order to have biomass in large quantities.

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Authors and Affiliations

  1. Tecnológico Nacional de México/Instituto Tecnológico de Tuxtla Gutiérrez, Tuxtla Gutiérrez, Chiapas, México

    Peggy Elizabeth Alvarez-Gutiérrez, Luis Alberto Ruiz-Ramirez & Federico Antonio Gutiérrez-Miceli

  2. CONACYT-TecNM-Instituto Tecnológico de Tuxtla Gutiérrez, Tuxtla Gutiérrez, Chiapas, México

    Peggy Elizabeth Alvarez-Gutiérrez

  3. Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán A.C., Mérida, Yucatán, México

    Gregorio del Carmen Godoy-Hernández

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  1. Peggy Elizabeth Alvarez-Gutiérrez
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  2. Luis Alberto Ruiz-Ramirez
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  3. Gregorio del Carmen Godoy-Hernández
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  4. Federico Antonio Gutiérrez-Miceli
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Correspondence to Gregorio del Carmen Godoy-Hernández or Federico Antonio Gutiérrez-Miceli .

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  1. Department of Botany, M.L.Sukhadia university, Udaipur, Rajasthan, India

    Kishan Gopal Ramawat

  2. Department of Pharmaceutical Botany, Jagiellonian University, Medical College, Kraków, Poland

    Halina Maria Ekiert

  3. Amarillo, TX, USA

    Shaily Goyal

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Alvarez-Gutiérrez, P.E., Ruiz-Ramirez, L.A., Godoy-Hernández, G.d.C., Gutiérrez-Miceli, F.A. (2019). Bonediol Production in Bonellia macrocarpa Hairy Root Culture. In: Ramawat, K., Ekiert, H., Goyal, S. (eds) Plant Cell and Tissue Differentiation and Secondary Metabolites. Reference Series in Phytochemistry. Springer, Cham. https://doi.org/10.1007/978-3-030-11253-0_24-1

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