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The Elucidation and Metabolic Engineering of Terpenoid Indole Alkaloid Pathway in Catharanthus roseus Hairy Roots

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Transgenesis and Secondary Metabolism

Part of the book series: Reference Series in Phytochemistry ((RSP))

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Abstract

Catharanthus roseus (Madagascar periwinkle) produces many pharmaceutically important chemicals such as vinblastine, vincristine, serpentine, and ajmalicine. They are synthesized through the highly branched and complex terpenoid indole alkaloid (TIA) pathway in C. roseus. Among the compounds produced in this pathway, vinblastine and vincristine are efficient anticancer drugs widely used in the clinic and are only found in C. roseus. Due to low accumulation of these TIAs within the plant and the infeasibility of production using chemical synthesis at industrial scale, the market price of these drugs remains high. In addition, inconsistent production along with increased demand causes these drugs to often show up on the FDA drug shortage list. With advanced knowledge of molecular biology, metabolic engineering, and bioinformatics, the construction of a robust and efficient alternative production platform in C. roseus hairy roots by manipulating the TIA pathway has become a promising strategy in recent years. In addition, recent advances in high-throughput sequencing technology have greatly sped up the elucidation of the TIA pathway. This review highlights present efforts to discover TIA pathway genes, their compartmentalization, and regulation in C. roseus and summarizes TIA pathway engineering efforts in C. roseus hairy roots.

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Authors and Affiliations

  1. Chemical and Biological Engineering Department, Colorado State University, 1370 Campus Delivery, Fort Collins, CO, 80523, USA

    Jiayi Sun & Christie A. M. Peebles

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  1. Jiayi Sun
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  2. Christie A. M. Peebles
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Correspondence to Jiayi Sun or Christie A. M. Peebles .

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  1. Department of Botany, Centre of Advanced Study, University of Calcutta, Kolkata, India

    Sumita Jha

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Sun, J., Peebles, C.A.M. (2017). The Elucidation and Metabolic Engineering of Terpenoid Indole Alkaloid Pathway in Catharanthus roseus Hairy Roots. In: Jha, S. (eds) Transgenesis and Secondary Metabolism. Reference Series in Phytochemistry. Springer, Cham. https://doi.org/10.1007/978-3-319-28669-3_14

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