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Modern spectrophotometers are usually accurate and reliable, but this should never be taken for granted, and there is a range of protocols that can be used to assess such parameters as wavelength accuracy (e.g., using holmium perchlorate solution or the sharp emission lines from a hydrogen or deuterium lamp), stray radiation, photometric accuracy (e.g., using potassium dichromate solution, noting the associated hazards), and baseline flatness. This is a routine requirement for an accredited testing laboratory, which may, for example, be required to comply with AS ISO/IEC 17025:2018, General Requirements for the Competence of Testing and Calibration Laboratories (Standards Australia: AS ISO/IEC 17025:2018(2018)), and is a requirement of Good Laboratory Practice (OECD 1998). It may also be essential for a forensic testing laboratory, in which all aspects of the laboratory’s testing practice may attract intense scrutiny, and...
References
ASTM International: ASTM E131-10 (2015) Standard terminology relating to molecular spectroscopy. ASTM International, West Conshohocken
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ASTM International: ASTM E169-16 (2016) Standard practices for general techniques of ultraviolet–visible quantitative analysis. ASTM International, West Conshohocken
ASTM International: ASTM E275-08 (2013) Standard practices for describing and measuring performance of ultraviolet, visible, and near-infrared spectrophotometers. ASTM International, West Conshohocken
ASTM International: ASTM E925-09 (2014) Standard practice for monitoring the calibration of ultraviolet–visible spectrophotometers whose spectral slit width does not exceed 2 nm. ASTM International, West Conshohocken
ASTM International: ASTM E958-13 (2013) Standard practice for measuring practical spectral bandwidth of ultraviolet–visible spectrophotometers. ASTM International, West Conshohocken
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© 2018 European Biophysical Societies' Association (EBSA)
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Wormell, P. (2018). Absorbance Spectroscopy: Quality Assurance. In: Roberts, G., Watts, A. (eds) Encyclopedia of Biophysics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-35943-9_773-1
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DOI: https://doi.org/10.1007/978-3-642-35943-9_773-1
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