The preparation of heavy-atom derivatives of protein crystals for use in multiple isomorphous replacement and anomalous scattering

Abstract

In this chapter an introduction to the heavy-atom data bank is provided and strategies are reviewed that can be adopted in the preparation of heavy-atom derivatives of protein crystals for use in multiple isomorphous replacement and anomalous scattering. Topics covered include: heavy-atom derivatives and their complexes; amino acids as ligands; the protein chemistry of heavy-atom reagents; metal-ion replacement in metalloproteins; analogues of amino acids; and the use of the heavy-atom data bank to select derivatives. This chapter is also available as HTML from the International Tables Online site hosted by the IUCr.